Development of SCAR Marker for Identification of the Perilla Species

• 발행기관: 한국육종학회
• 저자명: Myoung-Hee Lee Ki-Woong Yang Tae Joung Ha Chan-Sik Jung Suk-Bok Pae Chung-Dong Hwang Chang-Hwan Park In-Youl Baek Hyeon-Kyeong Kim Soon-Ki Park
• 간행물 정보: 『한국육종학회지』Vol.43 No.4, 186~193쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2011.08.30

영문 초록

This study is to generate SCARs markers for identification of Perilla species. A SCAR is a genomic DNA fragment at a single genetically defined locus that is identified by PCR amplification using a pair of specific oligonucleotide primers. We derived SCARs by sequencing and cloning the both ends of the amplified products of RAPD markers. Sixteen sequence-specific primers were synthesized from eight RAPD markers, which were completely sequenced. We developed the species-specific SCAR markers which could be used successfully in detecting genetic variation in four Perilla species. These markers could be used to verify species-origins of various forms of Perilla germplasms.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

REFERENCES

키워드

Perilla frutescens Molecular marker Random Amplification of Polymorphic DNA (RAPD) Sequence Characterized Amplified Regions(SCARs)