Optimization of Agrobacterium-mediated Transformation in Japonica-type Rice Oryza sativa L. cv. Dongjin for high Efficiency

• 발행기관: 한국육종학회
• 저자명: Se-Youn Han Dongjin Shin Seok-Jun Moon Seon-A Jeon Myung-Ok Byun Beom-Gi Kim
• 간행물 정보: 『한국육종학회지』Vol.44 No.3, 221~228쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2012.09.30

영문 초록

Plant transformation systems have been developed using several different approaches, and the development of reliable and efficient transformation systems is still one of the most important breakthroughs in molecular biology and biotechnology of rice. However, there are many cultivars in rice and transformation efficiency is dependent on the cultivar. The conventional Agrobacterium-mediated rice transformation system using secondary calli requires 14-16 weeks for establishing transgenic plantlets, and it is thought that somaclonal variation through the Tos17 retrotransposon occurs during the tissue culture stage. Here, we modified an Agrobacterium-mediated transformation system in rice cultivar Dongjin, adding an endosperm removal step that is critical to transformation frequency and changing the Agrobacterium strain and media composition. This method increased transformation efficiency and stability. These results will provide valuable information for scientists to use a reliable and efficient rice transformation system.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

REFERENCES

키워드

Rice transformation Scutellum Agrobacterium Somaclonal variation