Suppression Subtractive Hybridization을 통한 Venturia Nashicola 접종후 ‘93-3-98’에서 유도된 저항성 후보 유전자 선발

• 영문명: Candidate Gene Selection Induced by ‘93-3-98’ Pear after Inoculation of Venturia Nashicola by Suppression Subtractive Hybridization
• 발행기관: 한국육종학회
• 저자명: 천재안(Jae-An Chun) 남가영(Ga-Young Nam) 김세희(Se-Hee Kim) 조강희(Kang-Hee Cho) 김대현(Dae-Hyun Kim) 최인명(In-Myeong Choi) 신일섭(Il-Sheob Shin)
• 간행물 정보: 『한국육종학회지』Vol.47 No.1, 1~10쪽, 전체 10쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2015.03.30

영문 초록

Suppression subtractive hybridization was carried out to identify resistant genes against to pear scab caused by Venturia nashicola using leaves harvested at 24 and 48 hours after inoculation into ’93-3-98’ (highly resistant) and ‘Sweat Skin’ (highly susceptible). As a result of the ESTs analysis, nine and 14 unique genes were expressed on 24H (tester, mRNA at 24hr after inoculation of ’93-3-98’; driver, one of ‘Sweet Skin’) and 48H (tester, mRNA at 48hr after inoculation of ’93-3-98’; driver, one of ‘Sweet Skin’), respectively and genes related to defense or stress response were accounted for 40% (24H) and 42% (48H). Differential expressed genes classifying into PR protein family were pathogenesis-related protein 1a, major allergen Pyr c1 and allergen mal d 1 at 24H and major allergen Mal d 1.03B at 48H, respectively. Major allergen Pyr c1, F-Box/kelch-repeat protein, Flavoprotein wrbA, and hypothetical protein POPTRDRAFT_783792 are expected to closely connecting to scab resistance of pear following strongly expressed in highly resistant cv. ‘Bartlett’ and ‘93-3-98’ compared with moderately susceptible cv. ‘Gamcheonbae’, susceptible cv. ‘Wonhwang’, and highly susceptible cv. ‘Niitaka’ and ‘Sweat skin’.

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서 언

재료 및 방법

결과 및 고찰

REFERENCES

키워드

Pyrus Scab resistance Differential expressed gene Expression analysis Suppression subtractive hybridization