Kuehne의 LD50 계산법을 활용한 Salmonella Enteritidis Bacteriophage type(PT) 균주 상호간의 병원성 비교

• 영문명: Comparison of Virulence of Salmonella Enteritidis PT Isolates Using the Kuehne’s LD50 Determination Method
• 발행기관: 한국산업기술융합학회(구. 산업기술교육훈련학회)
• 저자명: 우용구(Yong-Ku Woo)
• 간행물 정보: 『산업기술연구논문지』산업기술교육훈련논문지 제22권 1호, 9~14쪽, 전체 6쪽
• 주제분류: 공학 > 산업공학
• 파일형태: PDF
• 발행일자: 2017.03.30

영문 초록

This study was evaluated for the efficiency of the rapid determination method of Log10 50% Lethal Doses(LD50) based on the comparison of virulence among Salmonella Enteritidis(SE) Bacteriophage type(PT) isolates. Kuehne’s method, which was not only simple and rapid but also ethical or animal welfare, was examined. A 10-fold serial dilutions of bacteria or virus were administered to six animals per groups. Above all, this method required the minimum number of animals for the determination of LD50 values than that of traditional ones and considered as more suitable for the present clinical laboratory situations in Korea, because of enforcement of the National Animal Welfare Raw. According to the 2 weeks old chicken challenge results based on the Kuehne’s method, two isolates(SEPT 1 & 4) were even more virulent than that of SEPT 6A strain. As a results, Kuehne’s method was found as a very rapid and simple for calculation of Log10 LD50 values and also an animal welfare.

목차

Ⅰ. 서 론

Ⅱ. 재료 및 방법

Ⅲ. 실험결과 및 고찰

Ⅳ. 결 론

키워드

LD50(50% 치사량) Salmonella Enteritidis(SE) Animal Welfare Raw(동물복지법) Challenge Experiment(공격접종) Virulence(침습성/병원성)