전장유전체수준 메틸레이션 분석을 통한 두경부암 특이 메틸레이션 바이오마커의 발굴

• 영문명: Genome-wide Methylation Analysis and Validation of Cancer Specific Biomarker of Head and Neck Cancer
• 발행기관: 대한두경부종양학회
• 저자명: 장재원(Jae Won Chang) 박기완(Ki Wan Park) 홍소혜(So Hye Hong) 정승남(Seung Nam Jung) 류려화(Li hua Liu) 김진만(Jin Man Kim) 오태정(Tae jeong Oh) 구본석(Bon Seok Koo)
• 간행물 정보: 『대한두경부종양학회지』제33권 제1호, 21~29쪽, 전체 9쪽
• 주제분류: 의약학 > 종양학
• 파일형태: PDF
• 발행일자: 2017.05.30

영문 초록

Methylation of CpG islands in the promoter region of genes acts as a significant mechanism of epigenetic gene silencing in head and neck squamous cell carcinoma (HNSCC). DNA methylation markers are particularly advantageous because DNA methylation is an early event in tumorigenesis, and the epigenetic modification, 5-methylcytosine, is a stable mark. In the present study, we assessed the genome-wide preliminary screening and were to identify novel methylation biomarker candidate in HNSCC. Genome-wide methylation analysis was performed on 10 HNSCC tumors using the Methylated DNA Isolation Assay (MeDIA) CpG island microarray. Validation was done using immunohistochemistry using tissue microarray of 135 independent HNSCC tumors. In addition, in vitro proliferation, migration/invasion assays, RT-PCR and immunoblotting were performed to elucidate molecular regulating mechanisms. Our preliminary validation using CpG microarray data set, immunohisto-chemistry for HNSCC tumor tissues and in vitro functional assays revealed that methylation of the Homeobox B5 (HOXB5) and H6 Family Homeobox 2 (HMX2) could be possible novel methylation biomarkers in HNSCC.

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Abstract

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대상 및 방법

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키워드

전유전체메틸레이선분석 메틸레이션 분자표지자 두경부암 Genome-wide methylation analysis Methylation biomarker Homeobox B5 (HOXB5) H6 Family Homeobox 2 (HMX2) Head and neck squamous cell carcinoma (HNSCC)