벤지딘으로 오염된 음용수를 섭취한 흰쥐에서 간장세포와 방광세포에 형성된 발암물질- DNA adduct에 관한 연구

• 영문명: Carcinogen-DNA adducts in Liver and Bladder of Sprague-Dawley rats treated with benzidine in drinking water
• 발행기관: 한국환경보건학회
• 저자명: 이진헌 신호상 장미선 홍춘표 최석남
• 간행물 정보: 『한국환경보건학회지』제27권 제3호, 63~70쪽, 전체 8쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2001.09.30

영문 초록

To identify and evaluate the benzidine-DNA adducts in liver and bladder, we exposed the 80 ppm benzidine to 40 sprague-dawley rats by drinking water for 4 weeks(6.2 mg/kg body wt./day). Only one benzidine-DNA adduct was found at the same site of thin layer chromatogram of 32P-postlabeling method in the liver and bladder of exposed rats. So we know the DNA adduct formed at liver and bladder were similar to each other, which was N-(deoxyguanosin-8-yl)-N -acetylbenzidine. Relative adduct labeling(RAL) of DNA adduct was similar to each other for 1 and 2 weeks, but that in liver was significantly higher than in bladder for 3 and 4 weeks. RALX109 of DNA adduct were 84.45 ±11.31 and 152.8 ±5.53 in liver, and were 24.76 ±7.06 and 38.00 ±10.57 in bladder for 3 and 4 weeks, respectively. Regression equation between liver and bladder was Y=-3.801+2.507 X(r=0.6036, p<0.01). In conclusion, benzidine-DNA adduct formed in liver was significantly higher than that in bladder, with the similar compound structure in sparague-dewley rates treated benzidine in drinking water.

키워드

Benzidine DNA adduct N-(deoxyguanosin-8-yl)-N -acetylbenzidine Relative adduct labeling RAL Liver Bladder