표준입도 분산조건에서 나노입자의 THP-1 세포에 대한 독성영향

• 영문명: Cytotoxicity of Nanoparticles in cultured THP-1 cells under the Standardized Condition of Size Distribution
• 발행기관: 대한약학회
• 저자명: 이한들(Handule Lee) 박주영(Juyoung Park) 박광식(Kwangsik Park)
• 간행물 정보: 『약학회지』제61권 제2호(2017년), 109~116쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2017.04.30

영문 초록

Effect of nanoparticles on cellular toxicity can be influenced by size and/or size distribution of the tested nanoparticles. Therefore, it is necessary to fulfill the requirement of standardized dispersion when toxicity potencies of different types of nanoparticles are compared. In this study, probe-sonicator calibration for delivered acoustic power was performed according to the NANoREG protocol for standardized size-distribution of test nanoparticles including silica nanoparticles (SiNP), ceria nanopaaticles (CeNP), titania nanoparticles (TiNP), silver nanoparticles (AgNP) and zinc nanoparticles(ZnNP). Based on the protocol, the increase of water temperature by sonication power was monitored and Pac value was obtained. When the time of 8 minutes and 25% amplitude calculated from the Pac value was applied to the sonication of silica nanoparticles, size distribution reached to the effective level, 210 nm < size < 270 nm, Polydispersity index(PDI) < 0.46. The same acoustic power used in the dispersion of silica nanoparticles was applied to the other test nanoparticles, and cytotoxicity test was performed by MTS assay in cultured THP-1 cells treated with nanoparticles for 24 hrs. As results, Lethal Concentration 50% (LC50) of silver nanoparticles was 78.44 ppm, which was most toxic among the test nanoparticles. The toxicity compared by the LC50 seemed to be AgNP > SiNP > TiNP > ZnNP > CeNP.

목차

실험방법

실험결과

고찰 및 결론

References

키워드

NANoREG sonication calibration nanoparticles size distribution