두경부암 세포주에서 TPEF 유전자의 methylation 변이

• 영문명: DNA METHYLATION OF TPEF GENE IN HEAD AND NECK SQUAMOUS CELL CARCINOMA CELL LINES
• 발행기관: 대한구강악안면외과학회
• 저자명: 전소영(So-Young Chun) 김정옥(Jung-Ock Kim) 홍수형(Su-Hyung Hong) 정유경(Yu-Kyung Chung) 장현중(Hyun-Jung Jang) 손윤경(Yoon-Kyung Shon) 김정완(Jung-Wan Kim)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제31권 제6호, 468~473쪽, 전체 6쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2005.12.30

영문 초록

Head and neck squamous cell carcinoma (HNSCC) is the sixth most common malignancy worldwide. The molecular mechanisms involved in the development and progression of these carcinomas are not well known. Abnormalities of genomic methylation patterns have been attributed a role in carcinogenesis and local de novo methylation at tumor suppressor loci was held to be involved in silencing of tumor suppressor genes. Using Ms APPCR, we previously isolated a hypermethylated fragment corresponded to the 5’end of TPEF gene from primary liver and lung cancer cells. To confirm the inactivation of TPEF gene by hypermethylation in HNSCC, we investigated correlation between methylation pattern and expression of TPEF in 10 HNSCC cell lines. In methylation analysis such as combined-bisulfite restriction analysis(COBRA) and bisulfite sequencing, only RPMI 2650 showed none methylated pattern and another 9 cell lines showed dense methylation. The TPEF gene expression level analysis using RT-PCR showed that these 9 cell lines had not or significantly low expression levels of TPEF as compared with RPMI 2650. In addition, the increase of TPEF reexpression by 5-AzaC as demethylating agent in 9 cell lines also indicated that TPEF expression was regulated by hypermethylation. These results of this study demonstrate that epigenetic silencing of TPEF gene by aberrant methylation could play an important role in HNSCC carcinogenesis.

키워드

Head and neck squamous cell carcinoma (HNSCC) DNA methylation Combined-bisulfite restriction analysis(COBRA)