구강암 세포주에 대한 CKD-602의 항암 효과

• 영문명: ANTICANCER EFFECT OF CKD-602(BELOTECAN, CAMTOBELL) ON THE ORAL CANCER CELL LINES
• 발행기관: 대한구강악안면외과학회
• 저자명: 윤필영(Pil-Young Yun) 옥용주(Yong-Ju Ok) 명훈(Hoon Myoung) 이종호(Jong-Ho Lee) 김명진(Myung-Jin Kim)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제31권 제1호, 7~12쪽, 전체 6쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2005.02.28

영문 초록

Purpose: CKD-602, a newly developed water-soluble campthotecin analogue, is a anticancer agent which act as a DNA topoisomerase I inhibitor. CKD-602 is known as more potent and tolerable agent. The main purposes of this study were to measure the cytotoxic effect of CKD-602 on the oral cancer cell lines and to evaluate the apoptotic aspect of dead cells. Materials and Methods: To determine the cytotoxic effect of CKD-602 on the oral cancer cell lines in comparison with various cell lines, such as lung cancer and colon cancer cell lines, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay was performed. And apoptosis was analyzed using fluorescence-activated cell sorting(FACS) system. Results: CKD-602 decreased the viability of malignant cells in a dose dependent manner and in a time dependent manner. CKD-602 showed excellent cytotoxicity to the oral cancer cell lines. Also, apoptotic portion was increased in a dose dependent manner. Conclusion: These findings indicated that CKD-602 induced apoptotic cell death in the various cell lines including oral cancer cell lines. From the results, it was suggested that CKD-602 would be a potential therapeutic agent for the oral cancer. More successive researches on the anticancer effect of CKD-602 should be performed.

키워드

CKD-602 Camptothecin Topoisomerase-I inhibitor Oral cancer Apoptosis