Isolation of human mesenchymal stem cells from the skin and their neurogenic differentiation in vitro

• 영문명: Isolation of human mesenchymal stem cells from the skin and their neurogenic differentiation in vitro
• 발행기관: 대한구강악안면외과학회
• 저자명: Jun-Ho Byun Eun-Ju Kang Seong-Cheol Park Dong-Ho Kang Mun-Jeong Choi Gyu-Jin Rho Bong-Wook Park
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제38권 제6호, 343~353쪽, 전체 11쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2012.12.30

영문 초록

Objectives: This aim of this study was to effectively isolate mesenchymal stem cells (hSMSCs) from human submandibular skin tissues (termed hSMSCs) and evaluate their characteristics. These hSMSCs were then chemically induced to the neuronal lineage and analyzed for their neurogenic characteristics in vitro. Materials and Methods: Submandibular skin tissues were harvested from four adult patients and cultured in stem cell media. Isolated hSMSCs were evaluated for their multipotency and other stem cell characteristics. These cells were differentiated into neuronal cells with a chemical induction protocol. During the neuronal induction of hSMSCs, morphological changes and the expression of neuron-specific proteins (by fluorescence-activated cell sorting [FACS]) were evaluated. Results: The hSMSCs showed plate-adherence, fibroblast-like growth, expression of the stem-cell transcription factors Oct 4 and Nanog, and positive staining for mesenchymal stem cell (MSC) marker proteins (CD29, CD44, CD90, CD105, and vimentin) and a neural precursor marker (nestin). Moreover, the hSMSCs in this study were successfully differentiated into multiple mesenchymal lineages, including osteocytes, adipocytes, and chondrocytes. Neuron-like cell morphology and various neural markers were highly visible six hours after the neuronal induction of hSMSCs, but their neuron-like characteristics disappeared over time (24-48 hrs). Interestingly, when the chemical induction medium was changed to Dulbecco s Modified Eagle Medium (DMEM) supplemented with fetal bovine serum (FBS), the differentiated cells returned to their hSMSC morphology, and their cell number increased. These results indicate that chemically induced neuron-like cells should not be considered true nerve cells. Conclusion: Isolated hSMSCs have MSC characteristics and express a neural precursor marker, suggesting that human skin is a source of stem cells. However, the in vitro chemical neuronal induction of hSMSC does not produce long-lasting nerve cells and more studies are required before their use in nerve-tissue transplants.

목차

I. Introduction

II. Materials and Methods

III. Results

IV. Discussion

V. Conclusion

키워드

Skin Mesenchymal stem cell In vitro neuronal differentiation