Effect of recombinant human bone morphogenetic protein-2 on bisphosphonate-treated osteoblasts

• 영문명: Effect of recombinant human bone morphogenetic protein-2 on bisphosphonate-treated osteoblasts
• 발행기관: 대한구강악안면외과학회
• 저자명: Taek-Kyun Kwon Jae-Min Song In-Ryoung Kim Bong-Soo Park Chul-Hoon Kim In-Kyo Cheong Sang-Hun Shin
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제40권 제6호, 291~296쪽, 전체 6쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2014.12.30

영문 초록

Objectives: Bisphosphonate-related osteonecrosis of the jaw (BRONJ) is a side effect of bisphophonate therapy that has been reported in recent years. Osteoclastic inactivity by bisphosphonate is the known cause of BRONJ. Bone morphogenetic protein-2 (BMP-2) plays an important role in the development of bone. Recombinant human BMP-2 (rhBMP-2) is potentially useful as an activation factor for bone repair. We hypothesized that rhBMP-2 would enhance the osteoclast-osteoblast interaction related to bone remodeling. Materials and Methods: Human fetal osteoblast cells (hFOB 1.19) were treated with 100 μM alendronate, and 100 ng/mL rhBMP-2 was added. Cells were incubated for a further 48 hours, and cell viability was measured using an MTT assay. Expression of the three cytokines from osteoblasts, receptor activator of nuclear factor-κB ligand (RANKL), osteoprotegerin (OPG), and macrophage colony-stimulating factor (M-CSF), were analyzed by real-time polymerase chain reaction and enzyme-linked immunosorbent assay. Results: Cell viability was decreased to 82.75%±1.00% by alendronate and then increased to 110.43%±1.35% after treatment with rhBMP-2 (P<0.05, respectively). OPG, RANKL, and M-CSF expression were all decreased by alendronate treatment. RANKL and M-CSF expression were increased, but OPG was not significantly affected by rhBMP-2. Conclusion: rhBMP2 does not affect OPG gene expression in hFOB, but it may increase RANKL and M-CSF gene expression.

목차

I. Introduction

II. Materials and Methods

III. Results

IV. Discussion

V. Conclusion

키워드

Bone morphogenetic protein-2 Alendronate Osteoblasts Macrophage colony-stimulating factor RANK ligand