두경부 편평상피세포암주에서 방사선 내성 관련 유전인자의 확인

• 영문명: Identifying Genes Related with Radiation Resistance in Oral Squamous Cell Carcinoma Cell Lines
• 발행기관: 대한두경부종양학회
• 저자명: 이세영(Sei Young Lee) 설정훈(Jeong Hun Seol) 박행란(Haeng Ran Park) 조남훈(Nam Hoon Cho) 최윤표(Yoon Pyo Choi) 라선영(Sun Young Rha) 백승재(Seung Jae Baek) 황준연(Jun Yeon Hwang) 김세헌(Se-Heon Kim)
• 간행물 정보: 『대한두경부종양학회지』제27권 제2호, 190~197쪽, 전체 8쪽
• 주제분류: 의약학 > 종양학
• 파일형태: PDF
• 발행일자: 2011.11.30

영문 초록

Background and Objective：Radiation resistance(RR) is one of main determinants of treatment outcome in oral squamous cell carcinoma(OSCC), but accurate prediction of RR is difficult. We aim to establish RR OSCC cell lines and identify genes related with RR by a measurement of altered gene expression after inducing RR. Material and Methods：OSCC cell lines, SCC15, SCC25 and QLL1, were treated with 2Gy radiation per session, and parts of them were alive in finally accumulated dosage of 60Gy through 30 times repetition of radiotherapy for inducing RR cell lines. We compared results of cDNA array and proteomics in non-radiated cell lines and RR cell lines to detect changes of gene expression. Western blot was used for the validation of results. Results：cDNA array revealed 265 commonly up-regulated genes and 268 commonly down-regulated genes in 3 RR cell lines comparing their non-radiated counterpart. Among them, 30 cancer related genes were obtained. Proteomics showed 51 commonly altered protein expressions in 3 RR cell lines and 18 cancer related proteins were obtained. Among the detected genes, we found NM23-H1 and PA2G4 were over-expressed in both cDNA array and pro-teomics. Western blot showed increased expression of NME1 in RR cell lines but not in PA2G4. Conclusion: We concluded that NM23-H1 may be a candidate of RR related gene and over-expression of NM23-H1 could be a biomarker to predict RR in OSCC.

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Squamous cell carcinoma Radioresistance cDNA arrays Proteomics 두경부편평세포암 방사선 저항성 cDNA array 프로테오믹스