생체 시료 중 아세틸콜린 및 콜린에 대한 효소-분광학적 정량분석

• 영문명: Enzymatic Spectrophotometric Determinations of Acetylcholine and Choline in the Biological Samples
• 발행기관: 대한약학회
• 저자명: 남명화(Myung Hwa Nam) 이성호(Sung-Ho Lee) 김기택(Ketack Kim) 한영희(Younghee Hahn)
• 간행물 정보: 『약학회지』제56권 제4호 (2012년), 222~229쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2012.08.31

영문 초록

In order to determine acetylcholine and choline in the biological samples, the specific enzymes of acetylcholinesterase (AChE) and choline oxidase (ChO), which utilize acetylcholine and choline as substrates, were employed to convert substrates to H2O2. The produced H2O2 was coupled to 4-aminoantipyrine/phenol with peroxidase (PO) yielding quinoneimine dye which was measured at 508 nm. In the present enzymatic spectrophotometric analysis the product at the equilibrium state was measured considering accuracy, precision, time and cost of the analysis. The developed analytical method yielded good linearity (calibration curve; A508=9534[acetylcholine]+0.009, correlation coefficient (R2); 0.999) with detection limit of 1.11×10-7 M, reasonable precision (relative standard deviation; 0.10~1.62% at 2.5×10-6 M~1.25×10-4 M) and accuracy (relative error; -0.24~0.97% at 4.13×10-6M~1.01×10-4 M) for acetylcholine chloride standard solution. The concentrations of acetylcholine and choline in human serum were found as 3.20×10-5 M and 1.14×10-4 M, respectively. The brain tissues of Sprague-Dawley strain rat contained 9.82 μg/g of acetylcholine and 6.53 μg/g of choline in the cerebrum, while 7.37 μg/g of acetylcholine and 5.34 μg/g of choline in the cerebellum.

키워드

acetylcholine choline enzymatic spectrophotometric analysis