토천궁 정유 성분의 수종 사람 암 세포주에 대한 세포 독성

• 영문명: Study on Cytotoxic Activities of the Essential Oil Compounds from Ligusticum chuanxiong against Some Human Cancer Strains
• 발행기관: 대한약학회
• 저자명: 심연(Youn Sim) 신승원(Seungwon Shin)
• 간행물 정보: 『약학회지』제55권 제5호 (2011년), 398~403쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2011.10.31

영문 초록

Ligusticum chuanxiong (Umbelliferae) is a perennial herb that has been used for invigoration of blood in Korean traditional medicine. It is especially important in gynecological therapy of amenorrhea and dysmenorrhea. In this study, the essential oil of L. chuanxiong was obtained by steam distillation and its main components of L. chuanxiong, Z-ligustilide and butylidene phthalide, were isolated by silica gel column chromatography. We investigated the cytotoxic effects of the essential oil fraction of L. chuanxiong and its main components on MCF-7, HeLa and SK-Hep-1 cell lines by measuring the number of surviving cancer cells after treatment through direct cell counting and MTT analysis, and by examining the morphological changes under the microscope. The essential oil from the rhizomes of L. chuanxiong and its main components showed significant cytotoxic activities for all three tested cell lines. We also observed morphological changes of shrinking and blebbing in the membranes of the three cell lines, depending on the concentration of L. chaunxiong oil or its main components.

키워드

Ligusticum chuanxiong essential oil Z-ligustilide butylidene phthalide MCF-7 HeLa SK-Hep-1 MTT morphology