돌연변이 마이오실린 발현에 의한 섬유주세포 사멸 및 그 기전

• 영문명: Mechanisms Underlying Trabecular Meshwork Cell Death Caused by Mutant Myocilin Expression
• 발행기관: 대한안과학회
• 저자명: 임동희 손성수 김태은 기창원,Dong Hui Lim, MD, Seongsoo Sohn, PhD, Tae Eun Kim, PhD, Changwon Kee, MD, PhD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume52,number12, 1507~1513쪽, 전체 7쪽
• 주제분류: 인문학 > 역사학
• 파일형태: PDF
• 발행일자: 2011.12.15

영문 초록

Purpose: To determine whether the expression of mutant myocilin can lead to death of human trabecular meshwork (HTM) cells and to determine whether the mechanism by which this occurs is apoptosis. Methods: HTM cells were transduced with a recombinant adenovirus expressing human mutant (Q368X) myocilin. The apoptotic death of HTM cells caused by expression of mutant myocilin was examined using a cell proliferation assay, flow cytometry, Western blot analysis, and immunocytochemistry. Results: It appeared that the expression of mutant myocilin itself was not sufficient to cause HTM cell death. Furthermore, the expression of mutant myocilin did not lead to apoptosis of HTM cells although it did elicit a protein unfolding response. Conclusions: Our data suggest that the mechanism of myocilin glaucoma is not apoptotic death of HTM cells caused by mutant myocilin expression, and that the actual mechanism remains unknown. J Korean Ophthalmol Soc 2011;52(12):1507-1513

키워드

Apoptosis, Myocilin, Primary open-angle glaucoma, Trabecular meshwork