산소로 유도된 망막증 모델에서 인간재조합 적혈구생성인자가 망막혈관에 미치는 영향

• 영문명: The Effect on the Retinal Vessels of rhEPO in a Rat Model of Oxygen-Induced Retinopathy
• 발행기관: 대한안과학회
• 저자명: 박창현 박태관 김성신 이승준 온영훈 김성운,Chang Hyun Park, M.D., Tae Kwann Park, M.D., Ph.D., Seung Joon Lee, M.D., Ph.D., Young Hoon Ohn, M.D., Ph.D., Sung Woon Kim, M.D., Ph.D.
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume48,number6, 835~844쪽, 전체 10쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2007.06.30

영문 초록

Purpose: To evaluate the effect of recombinant human erythropoietin (rhEPO) on the retinal vessels in a rat model of oxygen-induced retinopathy (OIR). Methods: Twenty-six Sprague-Dawley rat siblings were exposed to seven cycles each of hyperoxia (80% O2, 23.5 hours) and room air recovery (0.5 hours), followed by exposure to room air for five days. After each of the test group and control group was divided into two groups of rhEPO injected group and normal saline injected group, 400 u/kg of rhEPO or normal saline were intraperitoneally injected, respectively on the third, fifth, and eighth days of the experiment. Retinal neovascularization and capillary densities were compared between the rats on the eighth and fourteenth day after injection. The extent of VEGF164, and IGF-1 mRNA expression was measured in the rats on the 14th day post-injection. Results: Neovascularization and capillary density was the most increased in the rhEPO injected test group, but the avascular area was widely distributed in the vicinity of the retina. The degree of VEGF164 mRNA expression was highest in the test group that had been injected with normal saline. We found no difference in IGF-1 mRNA levels between the groups. Conclusions: We can infer that rhEPO can be a potent factor for inducing retinal neovascularization.

키워드

Erythropoietin, IGF-1, Neovascularization, OIR model, VEGF164