수정체 상피세포에서 TGF-β에 의한 MMP-2와 MMP-14의 발현과 조절 기전

• 영문명: The Regulation of MMP-2 and -14 Expressions by TGF-β in Lens Epithelial Cells
• 발행기관: 대한안과학회
• 저자명: 손민정 김종탁 주천기,Min Jung Son, B.S., Jong Tak Kim, Ph.D., Choun Ki Joo, M.D.
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume47,number7, 1110~1116쪽, 전체 7쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2006.07.31

영문 초록

Purpose: TGF-β is a key regulator of epithelial-mesenchymal transition. Among the TGF-β responses, cell migration is closely associated with the expression of matrix metalloproteinases (MMPs). Therefore, we determined which MMPs are regulated by TGF-β and examined the TGF-β signaling involved in this event, focusing on Src family tyrosine kinases (SFKs) Methods: First we examined the expression of MMPs in rat lens explant culture treated with TGF-β and LECs attached to the anterior capsules of patients with nuclear (N), anterior polar (AP) cataracts using RT-PCR and immunofluorescence staining. It was examined whether the expression of MMPs is regulated by SFKs. Results: The study using RT-PCR and immunofluorescence staining showed the expression of MMP-2 and -14 in explants and the expression of MMP-14 LECs of AP cataracts. The expression of MMP-2 and -14 was blocked by PP2 in explants. Furthermore, the activated form of SFKs was observed in LECs of AP cataracts by immunofluorescence staining. Conclusions: We suggest a novel role of SFKs signaling in the expression of MMP-14 induced by TGF-β.

키워드

Lens epithelial cell, Matrix metalloproteinase, Nuclear and anterior polar cataract, Transforming growth factor-β