단백체 분석을 위한 일차원 및 이차원 역상크로마토그래피의 비교

• 영문명: Comparison of 2-D RP-RP MS/MS with 1-D RP MS/MS for Proteomic Analysis
• 발행기관: 대한약학회
• 저자명: 문평곤(Pyong-Gon Moon) 조영은(Young-Eun Cho) 백문창(Moon-Chang Baek)
• 간행물 정보: 『약학회지』제54권 제5호 (2010년), 377~386쪽, 전체 10쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2010.10.31

영문 초록

Single-dimensional (1-D) and two-dimensional (2-D) LC methods were utilized to separate peptides from various sources followed by MS/MS analysis. Two-dimensional ultra-high performance liquid chromatography is a useful tool for proteome analysis, providing a greater peak capacity than 1-D LC. The most popular 2-D LC approach used today for proteomic research combines strong cation exchange and reversed-phase LC. We have evaluated an alternative mode for 2-D LC of peptides using 2-D RP-RP nano UPLC Q-TOF Mass Spectrometry, employing reversed-phase columns in both separation dimensions. As control experiments, we identified 129 proteins in 1-D LC and 322 proteins in 2-D LC from E.coli extract peptides. Furthermore, we applied this method to rat primary hepatocyte and a total of 170 proteins were identified from 1-D LC, and 527 proteins were identified from all 2-D LC system. The in-depth protein profiling established by this 2-D LC MS/MS from rat primary hepatocyte could be a very useful reference for future applications in regards to drug induced liver toxicity.

키워드

2-D LC MS/MS RP-RR hepatocyte