고속액체크로마토그라피법을 이용한 사람 혈장 중 알리벤돌(Alibendol)의 정량 및 검증

• 영문명: Determiniation and Validation of Alibendol using High Pressure Liquid Chromatography in Human plasma
• 발행기관: 대한약학회
• 저자명: 송현호(Hyun Ho Song) 유지영(Ji-young Yu) 김보겸(Bo Gyeom Kim) 박현주(Hyeon Ju Park) 최광식(Kwang Sik Choi) 권영이(Young Ee Kwon)
• 간행물 정보: 『약학회지』제54권 제4호 (2010년), 295~299쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2010.08.31

영문 초록

The aim of this study was to develop and validate for determination of alibendol in human plasma by HPLC method. After precipitation of 500 μl plasma samples by 50% methanol 50 μl and 60% perchloric acid 30 μl and the supernatant 50 μl was injected into HPLC. The assay was performed isocratically using 10 mM potassium phosphate (pH 3.0) and acetonitrile (80 : 20, v/v) as mobile phase. The C18 column (particle size 3.5 μm, 4.6×50 mm, Zorbax Eclipse) was used as a solid phase. The mobile phase was delivered at a flow-rate of 1.7 ml/min, detection was by ultraviolet absorption at 232nm and concentrations were calculated on the basis of peak areas. In these conditions, alibendol can be separated from ethylparaben, the internal standard, and endogenous substances. The retention times of alibendol and ethylparaben were just about 2.6 and 3.5 minutes, respectively. This rapid HPLC method was validated by examining the precision and accuracy for inter- and intra-day analysis. The standard curve was linear (R2=1.0000) over the concentration range of 0.05~20 μg/ml. The inter-day relative standard deviation (R.S.D.) and accuracy were 0.2~12.2% and 94.4~101.2% (82.7% at the lower limit of quatitation). The intra-day R.S.D. and accuracy were 0.1~11.8% and 98.8~102.5%, respectively. The method was successfully applied to the determination of alibendol in plasma for a pharmacokinetic study.

키워드

alibendol bioavailability bioequivalence human plasma HPLC/UV