랫트 간에서 tert-Butylhydroperoxide 투여에 의한 글루타치온과 타우린의 생합성 변화

• 영문명: Changes in Biosynthesis of Glutathione and Taurine in Rat Liver Challenged with tert-Butylhydroperoxide
• 발행기관: 대한약학회
• 저자명: 김선주(Sun Ju Kim) 박현아(Hyun Ah Park) 김영철(Young Chul Kim)
• 간행물 정보: 『약학회지』제53권 제6호 (2009년), 314~320쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2009.12.31

영문 초록

We examined metabolic conversion of cysteine into glutathione (GSH) and taurine in rat liver under oxidative stress. Administration of tert-butylhydroperoxide (t-BHP) into the portal vein of male rats resulted in a rapid elevation of serum sorbitol dehydrogenase, alanine aminotransferase, and aspartate aminotransferase activities, which decreased gradually in 24 hr. Hepatic cysteine concentration was reduced in 3 hr, and recovered progressively, reaching a level greater than 200% of the normal value in 24 hr. GSH was increased both in liver and blood at 9 hr after t-BHP challenge, whereas hypotaurine or taurine was not altered. γ-Glutamylcysteine synthetase (GCS) activity was increased from 9 hr after t-BHP treatment, but protein expression of the GCS-heavy subunit was not changed in liver. Activity or expression of cysteine dioxygenase was not affected by t-BHP treatment. Taken together, these data show that an acute oxidant challenge to the rats may induce upregulation of cysteine availability and GCS activity, resulting in an enhancement of hepatic GSH synthesis, but the increased cysteine level does not stimulate taurine synthesis via cysteine sulfinate pathway. It is indicated that the regulation of GSH and taurine biosynthesis from cysteine is not solely dependent on the cysteine concentration in rat liver under oxidative stress.

키워드

tert-butylhydroperoxide glutathione taurine y-glutamylcysteine synthetase cysteine dioxygenase