면역글로부린 G 생성에 대한 아이오타-카라기난의 어쥬번트 효과

• 영문명: Adjuvant Effects of Iota-Carrageenan on the Immunoglobulin G Production
• 발행기관: 대한약학회
• 저자명: 구본웅(Bon Woong Koo) 이광재(Kwang Jae Lee) 김만석(Man Suk Kim) 김하형(Ha Hyung Kim)
• 간행물 정보: 『약학회지』제44권 제6호 (2000년), 588~594쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2000.12.30

영문 초록

To investigate the effects of iota-carrageenan (CAR) and/or alum on the adjuvancity as well as the structural difference of oligosaccharide on the IgG2b in the adjuvant effect, C57BL/6 mice were immunized twice with fetuin as a model antigen. CAR alone showed no significant effect on induction of antibody except IgG1. In contrast, Alum-CAR (after mixing of antigen-Alum, CAR adjuvant was prepared) and CAR-Alum (after formulation of antigen-CAR, Alum adjuvant was prepared) enhanced production of antibody, especially, IgG2b. After separation of IgG2b, changes of glycosylation were investigated using enzyme-linked lectin assay. High affinity of IgG2b to N-acetylneuraminic acid, galactose and mannose-specfic lectin were induced by CAR-Alum adjuvant, however, the affinity of IgG2b induced by CAR-Alum to GlcNAc and GalNAc-specific lectin were much less than that induced by Alum-CAR.

키워드

Carrageenan adjuvant IgG production IgG2b glycosylation.