HPLC-DAD-MS/MS를 이용한 금은화 생리활성 물질의 동시분석

• 영문명: Simultaneous Analysis of Bioactive Metabolites from Lonicera japonica Flower Buds by HPLC-DAD-MS/MS
• 발행기관: 대한약학회
• 저자명: 유성광(Sungkwang Ryu) 전주은(Ju-eun Jeon) 강경원(Gyoung Won Kang) 강삼식(Sam Sik Kang) 신종헌(Jongheon Shin)
• 간행물 정보: 『약학회지』제52권 제6호 (2008년), 446~451쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2008.12.31

영문 초록

A high-performance liquid chromatography (HPLC) with diode array detector (DAD) and electrospray ionization mass spectrometry (ESI-MS) was established for the simultaneous determination of chlorogenic acid (1), sweroside (2), luteolin-7-O-glucoside (3), (E)-aldosecologanin (4) and 3,5-dicaffeoylquinic acid (5) from Lonicera japonica flower buds. The optimal chromatographic conditions were obtained on an ODS column (5 μm, 4.6×150 mm) with the column temperature 25℃. The mobile phase was composed of (A) water with 0.1% formic acid and (B) acetonitrile with 0.1% formic acid using a gradient elution, the flow rate was 0.3 ml/min. Detection wavelength was set at 250 nm. All calibration curves showed good linear regression (r2>0.994) within test ranges. The developed method provided satisfactory precision and accuracy with overall intra-day and inter-day variations of 0.05~1.95% and 0.15~2.26%, respectively, and the overall recoveries of 97.71~103.65% for the five compounds analyzed. The verified method was successfully applied to quantitative determination of the three types (phenolic compounds, iridoids and flavonoids) of bioactive compounds in 21 commercial L. japonica flower buds samples from different markets in Korea and China. The analytical results demonstrated that the contents of the five analytes vary significantly with sources.

키워드

HPLC-DAD Lonicera japonica flower buds iridoids phenolic compounds flavonoids