Benzo(a)pyrene 유도 DNA 손상에 대한 Genistein과 청국장추출물의 보호효과

• 영문명: Protective Effect of Genistein and Korean Fermented Soybean (Chungkookjang) Extract against Benzo(a)pyrene Induced DNA Damage in HepG2 Cells
• 발행기관: 대한약학회
• 저자명: 송은정(Eun Jeong Song) 김현표(Hyun Pyo Kim) 허문영(Moon Young Heo)
• 간행물 정보: 『약학회지』제52권 제5호 (2008년), 376~383쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2008.10.31

영문 초록

Chungkookjang (CKJ) is a fermented soybean product and one of favorite traditional foods in Korea. In this study, the alcoholic extract from Korean fermented soybean (CKJ) and its one of major flavonoids, genistein were evaluated for their protective effect against B(a)P induced cytotoxicity and DNA damage in HepG2 cells. CKJ extract and genistein decreased B(a)P-induced cell cytotoxicity. CKJ extract inhibited DNA single strand breaks evaluated by single cell gel electrophoresis. From RT-PCR study, it was revealed that CKJ extract decrease DNA damage induced in HepG2 cells expressing CYP1A1 and 1A2 by B(a)P. The metabolizing activities of CYP1A1 and CYP1A2, as measured by the 7-alkoxy resorufin O-deethylation (AROD) assay, showed that CKJ extract and genistein inhibited CYP1A1 and CYP1A2 activities. Genistein may contribute to these biological effects of CKJ extract at least in part. All these results indicate that CKJ extract and genistein may be useful for protection against B(a)P-induced cytotoxicity and DNA damage. Therefore, the alcoholic extract of Korean fermented soybean (CKJ) is suggested to be promising functional food which can prevent the cellular genotoxicity of dietary and lifestyle related carcinogens.

키워드

Korean fermented soybean Chungkookjang cytotoxicity DNA damage Comet assay RT-PCR CYP1A1 CYP1A2 enzyme catalytic assay