문주란 추출물의 HL-60 백혈병 세포 Apoptosis 유도 효과

• 영문명: Apoptosis Induction of HL-60 Leukemia Cells by Extract of Crinum asiaticum
• 발행기관: 대한약학회
• 저자명: 현재희(Jae Hee Hyun) 김엘비라(Elvira Kim) 강정일(Jung II Kang) 김상철(Sang Cheol Kim) 유은숙(Eun-Sook Yoo) 강희경(Hee-Kyoung Kang)
• 간행물 정보: 『약학회지』제52권 제1호 (2008년), 1~6쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2008.02.28

영문 초록

The present study investigated the antiproliferative effects of Crinum asiaticum var. japonicum against HL-60 human leukemia cells. The 80% MeOH extract or several solvent fractions from the C. asiaticum inhibited the growth of HL-60 cells, whereas the growth of HEL-299 cells, human embryonic lung fibroblast, was scarcely inhibited. When the HL-60 cells were treated with the CHCl3 fraction, the BuOH fraction, the EtOAc fraction and the H2O fraction, DNA ladder, chromatin condensation and increase of sub-G1 hypodiploid cells were observed. Furthermore, the CHCl3 fraction and the BuOH fraction reduced Bcl-2 mRNA level, whereas Bax mRNA level was increased. These results suggest that the inhibitory effect of C. asiaticum on the growth of the HL-60 cell might be mediated through the induction of apoptosis via the down-regulation of Bcl-2. Taken together, components of C. asiaticum might have a therapeutic potential for the treatment of human leukemia.

키워드

Crinum asiaticum HL-60 cell apoptosis