Oligonucleotide Microarray와 cDNA Microarray를 이용한 위암조직의 대단위 유전자 발현 비교

• 영문명: Comparison of Expression Profiling of Gastiric Cancer by Oligonucleotide and cDNA Microarrays
• 발행기관: 대한약학회
• 저자명: 정광화(Kwang Hwa Jung) 김정규(Jung Kyu Kim) 노지헌(Ji Heon Noh) 은정우(Jung Woo Eun) 배현진(Hyun Jin Bae) 이석형(Sug Hyung Lee) 박원상(Won Sang Park) 유남진(Nam Jin Yoo) 이정용(Jung Young Lee) 남석우(Suk Woo Nam)
• 간행물 정보: 『약학회지』제51권 제3호 (2007년), 179~185쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2007.06.30

영문 초록

Gastric cancer is one of the most common malignancies in Korea, but the predominant bolecular event underlying gastric carcinogenesis remain unknown. Recently, DNA microarray technology has enabled the comprehensive analysis of gene expression level, and as such has yielded great insight into the molecular nature of cancer. However, despite the powerful approach of this techniques, the technical artifacts and/or bias in applied array platform limited the liability of resultant tens of thousand data points from microarray experiments. Therefore, we applied two different array platforms, such as oligonucleotide microarray and cDNA microarray, to identify gastric cancer related large-scale molecular signature of the same human specimens. When thirty sets of matched human gastric cancer and normal tissues subjected to oligonucleotide microarray, total 623 genes were resulted as differently expressed genes in gastric cancer compared to normal tissues, and 252 genes for cDNA microarray analysis. In addition, forty three outlier genes which reflect the characteristic expression signature of gastric cancer beyond array platform and analytical protocol was recapitulated from two different expression profile. In conclusion, We were able to identify robust large-scale molecular changes in gastric cancer by applying two different platform of DNA microarray, this may facilitate to understand molecular carcinogenesis of gastric cancer.

키워드

cDNA microarray oligonucleotide microarray gastric cancer