백출 지상부의 항산화 성분

• 영문명: Anti-oxidative Compounds from The Aerial Parts of Atractylodes macrocephala Koidzumi
• 발행기관: 대한약학회
• 저자명: 한정훈(Jung Hoon Han) 김진효(Jin Hyo Kim) 김성건(Sung Gun Kim) 정성희(Sung Hee Jung) 김도훈(Do Hoon Kim) 김기은(Gi Eun Kim) 황완균(Wan Kyunn Whang)
• 간행물 정보: 『약학회지』제51권 제2호 (2007년), 88~95쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2007.04.30

영문 초록

Atractylodes macrocephala has been used for renal anorexia, gastroenteritis, cold, dyspepsia in Korean folk medicine. Specially aerial parts has been eaten as edible mountain herbs. In order to investigate the efficacy of antioxidant activity, the activity guided fractionation and isolation of physiologically active substance were performed. For the investigation of the active components from Atractylodes macrocephala MeOH extracts of aerial parts of Atractylodes macrocephala Koidzumi L. were suspended with H2O, partitioned by CHCl3. In order to investigate the efficacy of antioxidative activity, the activity guided fractionation and isolation of physiologically active substance were performed. CHCl3,, H2O, 30% MeOH, 60% MeOH, MeOH fractions were examined antioxidative activity by DPPH method. It was revealed that 30% MeOH and 60% MeOH fractions have significantly antioxidant activity. From 30% MeOH and 60% MeOH fraction, six flavonoids (7-methoxy-pinocembrin-7-O-β-D-glucopyranoside, apigenin-8-C-β-D-glucopyranoside, 4'-caffeoyl-luteolin-6-glucopyranoside, luteolin-6-C-β-D-glucopyranoside, apigenin-6-C-β-D-glucopyranoside, luteolin) and four phenylpropanoids (3-feruloylquinic acid 4,5-di-O-cafeoyquinic acid, feruloyl acid, 3,5-di-O-caffeoylquinic acid) were isolated. To investigate the antioxidant activities of each compounds, we measured radical scavening activity with DPPH method and anti-lipid peroxidative efficacy on low density lipoprotein (LDL) with TBARS assay. Six compounds (Ⅲ, Ⅳ, Ⅴ, Ⅵ, Ⅸ, Ⅹ) which hae antioxidant factor showed significant activities.

키워드

Atractylodes macrocephala DPPH method TBARS assay anti-lipid peroxidation