Ethaverine 화합물이 P815 세포중의 Serotonion 함량에 미치는 영향

• 영문명: Inhivitory Effects of Ethaverine on Serotonin Content in Muine Mastocytoma P815 cells
• 발행기관: 대한약학회
• 저자명: 김응일(Eung Il Kim) 신정수(Jung Soo Shin) 임성실(Sung Cil Lim) 박승국(Seung Kook Park ) 이명구(Myung Koo Lee)
• 간행물 정보: 『약학회지』제51권 제2호 (2007년), 83~87쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2007.04.30

영문 초록

The effects of ethaverine on serotonin biosynthesis in murine mastocytoma P815 cells were investigated. Ethaverine at 2.5~10μM decreased serotonin content in a concentration-dependent manner. The IC50 value of ethaverine was 6.1μM. Ethaverine at concentrations up to 20μM was not cytotoxic towards P815 cells. Under these conditions, tryptophan hydroxylase (EC 1.14.16.4; TPH), a rate-limiting enzyme in serotonin biosynthesis, was inhibited by ethaverine in P815 cells (15.3% inhibition at 7.5μM), however, aromatic L-amino acid decarboxylase (EC 4.1.1.28) was not. These results indicate that ethaverine leads to a decrease in serotonin content by reducing TPH activity in P815 cells.

키워드

ethaverine serotonin content tryptophan hydroxylase P815 cells