저산소 및 산소재도입시 vitamin C와 E가 간장 약물대사 기능에 미치는 영향

• 영문명: Effects of Vitamins C and E on Hepatic Drug Metabolizing Function in Hypoxia/Reoxygenation
• 발행기관: 대한약학회
• 저자명: 윤기욱(Ki Wook Yoon) 이상호(Sang Ho Lee) 이선미(Sun Mee Lee)
• 간행물 정보: 『약학회지』제44권 제3호 (2000년), 237~244쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2000.06.29

영문 초록

Liver isolated from 18 hours fasted rats was subjected to N2 hypoxia (for 45 min) followed by reoxygenation (for 30 min). The perfusion medium used was Krebs-Henseleit bicarbonate buffer (pH 7.4, 370C). Vitamin C (0.5mM) and trolox C (0.5mM), soluble vitamin E analog, were added to perfusate. Lactate dehydrogenase (LDH), total glutathione, oxidized glutathione, lipid peroxide and drug-metabolizing enzymes were measured. After hypoxia LDH significantly increased but this increase was attenuated by vitamin C and combination of vitamin C and E. Total glutathione and oxidized glutathione in perfusate markedly increased during hypoxia and this increase was inhibited by vitamins C, E and its combination. Similarly, oxidized glutathione and lipid peroxide in liver tissue increased after hypoxia and reoxygenation and this increase was inhibited by vitamin E and combination of vitamin C and E. Hepatic drug metabolizing function (phase I, II) were suppressed during hypoxia but improved during reoxygenation. While vitamins C and E only increased glucuronidation, the combination of vitamin C and E increased the oxidation, glucuronidation and sulfation. Our findings suggest that vitamins C and E synergistically ameliorates hepatocellular damage as indicated by abnormalities in drug metabolizing function during hypoxia/reoxygenation and that this protection is in major part, caused by decreased oxidative stress.

키워드

N2 hypoxia/reoxygenation drug metabolizing enzymes vitamin C trolox C.