Flavin mononucleotide (1,4-butanediamine) Pt(II) Complex와 Cisplatin의 세포주기에 대한 유세포 분석 및 ICR계 생쥐에서의 신장독성에 대한 생화학적 분석

• 영문명: Flow cytometry of cell-cycle on Flavin mononucleotide (1,4-butanediamine) Pt(II) Complex and Cisplatin and Their Biochemical Analysis of Nephrotoxicity in ICR Mice
• 발행기관: 대한약학회
• 저자명: 권영이(Y. E. Kwon) 황규자(K. J. Whang) 김안근(A. K. Kim) 김국환(K. H. Kim) 김원규(W. K. Kim) 안동춘(D. C. Ahn)
• 간행물 정보: 『약학회지』제44권 제2호 (2000년), 149~154쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2000.04.29

영문 초록

Flavin mononucleotide (1,4-butanediamine) Pt(II) complex (7FMN) was synthesized and screened anticancer activity [J.Pharm.Soc.Korea 43(6), 762-770 (1999)]. 7FMN have good water solubility and moderate anticancer activity. In this paper, cell-cycle specificity and nephrotoxicity were studied. Interaction of DNA with cisplatin and synthesized 7FMN was analyzed by flow cytometry, and showed G2 arrest in L1210 cell fine. It means that cell-cycle on L1210 was inhibit in S phase by cisplatin and 7FMN. In order to biochemically analyze nephrotoxicity of cisplatin and 7FMN, after injecting each agent intrapenritoneary, blood was exsangumated after 6 hours, 1 day, 3 days and 7 days, respectively. Then, serum was separated from the blood. The serum level of BUN, creatinine and uric acid in cisplatin and 7FMN administrated mice (25-35g, ICR strain, a dose each 8, 12 and 16 times of the IC50 value, cisplatin; 7 times) were determined by autochemistry analyzer. In cisplatin-administered mice group, BUN level was elevated than normal control group at 3rd day and repaired at 7th day. In 7FMN administrated group was not elevated. Crea@e and uric acid level were no difference with the normal control group. Therefore synthesized 7FMN is less toxic than cisplatin in nephrotoxicity.

키워드

Flavin mononucleotide(1,4-butanediamine) Pt(Ⅱ)complex anticancer activity flow cytometry nephrotoxicity.