부자류 생약의 성분인 아코니틴과 관련 알킬로이드의 정량

• 영문명: Determination of Aconitine and Related Alkaloids in Processed Buza
• 발행기관: 대한약학회
• 저자명: 엄동욱(Dong Ok Eom) 한상욱(Sang Wook Han) 신현덕(Hyun Duck Shin)
• 간행물 정보: 『약학회지』제44권 제2호 (2000년), 135~140쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2000.04.29

영문 초록

Determination of Aconitum alkaloids in processed Buza (Cho-0, Salted Buza, Moist-heating Buza, Limed Buza), which had been prepared from the raw tubers of Aconitum chiisanenseb(Ranunculaceae), was established using visible spectrophotometry and high-performance liquid chromatography (HPLC) method especially for Aconitine analysis. Aconitum alkloids were reacted with tetra- thiocyanatocobalt[II] complex ion to form a stable ion pair. The reaction product was insoluble in water but freely soluble in several organic solvents. 1.2-Dichloroethane was the best extracting solvent among the examined solvents. Spectrophotometry of Aconitum alkaloids at lambdamax 625 was carried out. The HPLC method for aconitine was carried out using Radial PAK-CN column with gradient solvent system by solvent mixture of acetonitrile and phosphate buffer (pH 3.0) at 40oC and lambda254nm. Linear relationship was found between absorbance response and concentration of aconitine in range of 0.45mM-0.9mM (r2=O.9949) by spectrophotometry and 0.3mM-1.2mM (r2=0.9983) by HPLC method. These methods have been found to be suitable and reproducible for routine analysis of Aconitum alkaloids and its pharmaceutical preparations.

키워드

Processed Buza aconitine Aconitum alkaloids determination spectrophotometry HPLC method.