db/db 마우스에서 상백피탕의 혈당강하 활성 및 기전연구

• 영문명: Blood Glucose Lowering Activity and Mechanism of Sangbackpitang (SBPT) in db/db Mouse
• 발행기관: 대한약학회
• 저자명: 이성현(Sung Hyun Lee) 안세영(Se Young An) 두호경(Ho Kyung Du) 정성현(Sung Hyun Chung)
• 간행물 정보: 『약학회지』제43권 제6호 (1999년), 818~826쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1999.12.28

영문 초록

Antidiabetic activity and mechanism of Sangbackpitang (SBPT) was examined in db/db mice, which is a spontaneously hyperglycemic, hyperinsulinemic and obese animal model. SBPT and acarbose were administered orally for 4 weeks. Fasting and non-fasting serum glucose, glycated hemoglobin and triglyceride were all reduced when compared between db/db control group and SBPT treated group. At 12th week after birth, SBPT increased an insulin secretion although statistic significance was not seen. Total activities of sucrase, maltase and lactase in SBPT treated group were all decreased when compared to db/db control. On the other hand, sucrase and maltase activities in acarbose treated groups were increased. Effect of SBPT on MRNA expression of glucose transporter(GLUT-4) was also examined. Quantitation of glucose transporter was performed by RT-PCR and in vitro transcription with co-amplification of rat -actin gene as an internal standard. Muscular GLUT-4 mRNA expression in SBPT treated group was increased significantly. These results may suggest that SBPT lowered blood glucose ascribing to inhibition of glycosidase-catalyzed reaction and upregulation of muscular GLUT-4 mRNA expression.

키워드

Sangbackpitang (SBPT) db/db mouse glycated hemoglobin triglyceride insulin glycosidase glucose transporter RT-PCR.