새로운 항암제 DA-125의 유전자 복제 억제 기작

• 영문명: Inhibitory Mechanism of a New Antitumor Agent DA125 on DNA Replication
• 발행기관: 대한약학회
• 저자명: 이상광(Snag Kwang Lee) 김도진(Do Jin Kim) 오유택(You Take Oh) 이상득(Sang Deuk Lee) 우은란(Eun Rhan Woo) 신차균(Cha Gyun Shin)
• 간행물 정보: 『약학회지』제43권 제5호 (1999년), 623~628쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1999.10.28

영문 초록

DA-125, a new antitumor agent, was compared with adriamycin, a known DNA intercalator, in terms of inhibitory mechanism of DNA replication by using replicating simian virus 40(SV40) genome In vivo. In analyzing the SV40 DNA replication intermediates present in cells treated with DA-125, it was not observed to accumulate B-dimers of SV40 DNA which are prominent in adriamycin-treated cells. However, treatment with DA-125 induced dose-dependent formation of DNA-topoisomerase complex which is characteristic of topoisomerase poisons. In addition, DA-125 showed more efficient in inhibiting SV40 DNA replication than adriamycin. Therefore, on the basis of this observation, we suggest that DA-125, a derivative of adriamycin, inhibits DNA replication by blocking topoisomerase activity as a topoisomerase poison although adriamycin blocks topoisomerase activity as a DNA intercalator.

키워드

Topoisomerase DA-125 adriamycin SV40 DNA replication poison.