황해쑥(Artemisia argyi)의 H9(ATCC HTB176)세포에 대한 세포독성 및 항산화효소 활성

• 영문명: Cytotoxicity of Artemisia argyi Extract Against H9(ATCC HTB176) Cell and Antioxidant Enzyme Activities
• 발행기관: 대한약학회
• 저자명: 김경하(Kyoung Ha Kim) 정대영(Dae Young Jung) 민태진(Tae Jin Min) 박시원(Sie Won Park)
• 간행물 정보: 『약학회지』제43권 제5호 (1999년), 598~605쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1999.10.28

영문 초록

The hot water and methanol extracts of Artemisia argyi showed considerable cytotoxicities against H9(ATCC HTB176) cancer cell with IC50 values of 48.6mcg/ml and 50.9mcg/ml, respectively. These cytotoxicities were found to be dependent on the extract concentrations and culture days. CuZnSOD and MnSOD activities were significantly increased in the cytoplam and mitochondria fractions of cancer cell, and media in the presence of Artemisia argyi. Such enhanced SOD activities were generally in the range of two to threefolds. In contrast to SOD, catalase and glutathione peroxidase activities were not detected at all. These results suggest that Artemisia argyi have generated O2- in the mitochondria and cytoplasm of H9 cancer cell with concurrent induction of CuZnSOD and MnSOD in situ, which dismutate O2- to H2O2. Without coordinated actions of catalase and/or gluathione peroxidase H2O2 is easily converted to very toxic OH and these reactive oxygen species together might have induced nectosis and/or apoptosis of H9 cell.

키워드

Artemisia argyi H9 (ATCC HTB 176) cytotoxicity superoxide superoxide dismutase(SOD) catalase glutathione peroxidase.