고등식물로부터 Chitin Synthase Ⅱ 활성 저해물질의 탐색

• 영문명: Screening of Chitin Synthase Ⅱ Inhibitors from Medicinal and Wild Plants
• 발행기관: 대한약학회
• 저자명: 황의일(Eui Il Hwang) 이향복(Hyang Bok Lee) 김성욱(Sung Uk Kim)
• 간행물 정보: 『약학회지』제43권 제4호 (1999년), 502~508쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1999.08.28

영문 초록

Chitin is an important structural component of fungal cell wall and is synthesized by chitin synthase Ⅰ, Ⅱ, and Ⅲ. The chitin synthase Ⅱ is an essential enzyme for the formation of primary septum in Saccharomyces cereviside. Therefore, specific inhibitors of this enzyme might block the formation of fungal cell wall and could be used as effective antifungal agents. To search chitin synthase Ⅱ inhibitors from natural products, 67 plants were extracted with methanol and examined for the inhibitory activities against chitin synthase Ⅱ of S. cerevisiae by our cell free assay system. As a result, the extracts from 16 plants showed more than 70% inhibition at the concentration of 280mcg/ml. Of note, Laurus nobilis (81.4%), Lonicera maackii (81.5%), Berchemia berchemiaefolia (82.9%), Koelreuteria paniculata (87.9%), Chamaecyparis pisifera (86%) and Taxus cuspidata (83.9%) inhibited strongly the chitin synthase Ⅱ activity.

키워드

Fungal cell wall chitin, chitin synthase Ⅱ inhibitors antifungal agents.