무청으로부터 분리된 이소람네틴 3-O-beta-D글루코피라노사이드의 항헤르페스 바이러스 1형(HSV-1) 효과

• 영문명: Anti-Herpes Simplex Virus type I (HSV-1) Effect of Isorhamnetin 3-0-beta-D-Glucopyranoside Isolated from Brassica rapa
• 발행기관: 대한약학회
• 저자명: 김호경(Ho Kyoung Kim) 강봉주(Bong Joo Kang) 박갑주(Kap Joo Park) 고병섭(Byoung Seob) 황완균(Wann Kyun Whang)
• 간행물 정보: 『약학회지』제42권 제6호 (1998년), 607~612쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1998.12.31

영문 초록

In the course of our search for anti-Herpes simplex virus type I (HSV 1) substances from natural sources, we screened crude drugs for their antiviral activity using SRB assay. The methanol extract from herb of Brassica rapa (Cruciferae) was found to inhibit HSV-1. Though bioassay-directed fractionation of the extract, anti-HSV-1 agent was isolated by chromatographic separation using Amberlite XAD-4 and Sephadex LH-20. The structure of compound I was elucidated by spectral means including 1H-1H COSY, HMQC and HMBC to be isorhamnetin 3-O-beta-D-glucopyranoside (compound I). Compound I was active against HSV-1 with the 50% effective concentration of O.42mg/ml and the 50% cytotoxicity of 5.0mg/ml.

키워드

Anti-Herpes simplex virus type 1(HSV-1) Cruciferae SRB assay Brassica rapa Isorhamnetin 3-O-β-D-glucopyranoside.