간 보호제 및 담즙산류들이 마크로파지 세포주에서 TNF-alpha 분비에 미치는 효과

• 영문명: Effect of Hepatoprotective Agents and Bile acids on TNF-A Production in Macrophage Cell Lines
• 발행기관: 대한약학회
• 저자명: 조재열(Jae Youl Cho) 박지수(Ji Soo Park) 유은숙(Eun Sook Yoo) 백경업(Kyong Up Baik) 박명환(Myung Hwan Park)
• 간행물 정보: 『약학회지』제42권 제1호 (1998년), 82~88쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1998.01.31

영문 초록

The effect of hepatoprotective agents and bile acids on tumor necrosis factor-alpha, (TNF-alpha) production in murine and human macrophage cell line (RAW264.7 and U937) was investigated. The hepatoprotective agents including silymarin and its major component, silybin, significantly inhibited TNF-alpha production in a concentration dependent manner (IC50 of silybin=67.7mcg/ml (140.3mcM)). In differentiated U937 cells, especially, silybin showed more effective inbitory activity (IC50=35.1mcg/ml (72.7mcM)). These results suggest that silymarin and silybin may inhibit TNF-alpha production in the process of hepatic diseases in human. However, biphenyldimethyl dicarboxylate (DDB) was not effective. In the case of bile acids, chenodeoxycholic acid (CDCA) showed a concentration dependent inhibitory effect on TNF-alpha production (IC50 of CDCA= 71.5mcg/ml (182.1mcM)). In contrast, glycine or taurine conjugated form (G-CDCA or T-CDCA) restored to the control level or significantly increased TNF-alpha production. And also ursodeoxycholic acid (UDCA) and its conjugated forms (G-UDCA and T-UDCA) showed a variety of patterns on TNF-alpha production by changes of functional groups and concentration. These results also indicate that bile acids may regulate TNF-alpha production in normal hepatic function or disease conditions.

키워드

TNF-α silymarin silybin biphenyldimethyl dicarboxylate ursodeoxycholic acid chenodeoxycholic acid.