해모필루스 K-12가 생산하는 황산전이효소의 고정화

• 영문명: Immobilization of Arylsulfate Sulfotransferase Obtained from Haemophilus K-12
• 발행기관: 대한약학회
• 저자명: 이남수(Nam Soo Lee) 김병택(Byung Taek Kim) 최승기(Seung Ki Choi) 김동현(Dong Hyun Kim)
• 간행물 정보: 『약학회지』제42권 제1호 (1998년), 53~58쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1998.01.31

영문 초록

A novel type of sulfotransferase, arylsulfate sulfotransferase (EC 2.8.2.22) purified from Haemophilus K-12, an intestinal bacterium of a mouse, was immobilized onto AH-Sepharose 4B, CH-Sepharose 4B and DEAE-celluose. The enzyme was stabilized for storage more markedly by covalent immobilization onto AH-Spharose 4B or CH-Sepharose 4B and by adsorptive immobilization onto DEAE-cellulose than the free enzyme. The optimal pH and acceptor substrate specificity of immobuized enzyme were similar to those of the free enzyme.

키워드

Sulfotransferase immobilized enzyme haemophilus K-12.