맥아당결합 단백질에 융합된 면역결핍 바이러스 인테그라제의 생산 및 분석

• 영문명: Production and Characterization of Human Immunodeficiency Virus Integrase Fused with a Maltose-Binding Protein
• 발행기관: 대한약학회
• 저자명: 김도진(Do Jin Kim) 오유택(You Take Oh) 신차균(Cha Gyun Shin)
• 간행물 정보: 『약학회지』제42권 제1호 (1998년), 46~52쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1998.01.31

영문 초록

Retroviral integrase is required for integration of viral DNA into the host cell chromosome. Human immunodeficiency virus type-1 integrase was partially purified as a part of a fusion protein linked to a maltose-binding protein and characterized in terms of an endonucleolytic activity. The concentration of the fusion protein purified through an amylose column was about 12mg/ml. Indicating that the solubility of the fusion protein is highly increased by the presence of a maltose-binding protein, considering that the integrase protein alone is poorly solubilized. The endonucleolytic activity of the fusion protein was detected at 0.1 to 1.OmM Mn++ ion, but not at any concentrations tested of Mg++ ion.

키워드

Fusion protein integrase maltose-binding protein endonucleolytic activity.