산소자유기에 의해 저해된 배양 척수감각 신경절 세포에 대한 상피세포성장인자의 영향

• 영문명: Effect of EGF against Oxygen Radical-induced Neurotoxicity in Cultured Spinal Dorsal Root Ganglion Neurons of Mouse
• 발행기관: 대한약학회
• 저자명: 박승택(Seung Taeck Park) 김형룡(Hyung Ryong Kim) 채한정(Han Jung Chae)
• 간행물 정보: 『약학회지』제41권 제1호 (1997년), 99~104쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1997.01.31

영문 초록

In order to elucidate the cytotoxic effect of oxygen radicals on cultured spinal dorsal root ganglion(DRG) neurons derived from mouse. the neurotoxic effect of oxygen radicals was examined after cultured DRG neurons were exposed to xanthine oxidase(XO) and hypoxanthine(HX)-oxygen radical generating system. In addition. neuroprotective effect of epidermal growth factor(EGF) against oxidant-induced neurotoxicity was also evaluated in these cultures. The results were, as follows: 1. Lethal concentration 50(LC50) was 35mU/ml XO and 0.1mM HX in cultured DRG neurons. 2. Oxygen radicals induced the morphological changes such as the decrease of cell number and loss of neurites in these cultures. 3. EGF increased the cell viability and neurofilament in neurons damaged by oxygen radicals. From above the results, it is suggested that oxygen radicals have a cytotoxic effect on cultured DRG neurons of neonatal mouse and selective neurotrophic factors such as EGF are, effective, in blocking the neurotoxicity induced by oxygen radicals in cultured spinal DRG neurons.

키워드

Spinal dorsal root ganglion Epidermal growth factor Xanthine oxidase hypoxanthine.