U-937 세포에서 세라마이드의 세포증식과 세포주기 조절단백질에 대한 작용

• 영문명: Effect of Ceramide on Cell Growth and Cell Cycle Related Proteins in U-937 Cells
• 발행기관: 대한약학회
• 저자명: 이재훈(Jae Hoon Lee) 최관수(Kwan Soo Choi) 김미영(Mie Young Kim)
• 간행물 정보: 『약학회지』제41권 제1호 (1997년), 94~98쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1997.01.31

영문 초록

Ceramide. a product of sphingomyelin hydrolysis, has been proposed as a lipid second messenger ymediating antiproliferative activation. In this study, we examined the role of the cell cycle-related proteins in the ceramide-mediated growth suppression. Treatment of U-937 cells with C2-ceramide(N-acetylsphingosine) resulted in growth suppression in a time- and concentration dependent manner. Ceramide induced concentration dependent dephosphorylation of retinoblastoma gene product (Rb). Rb remains hypophosphorylated in synchronized cells even after serum stimulation in the presence of ceramide. Ceramide decreased the expression of cyclin D1 and cyclin E levels. These results suggest that antiproliferative effect of ceramide is associated with hypophosphorylation of Rb and decreased expression of cyclin D1 and cyclin E.

키워드

ceramide growth suppression cell cycle Rb cyclin.