효소면역측정법 (ELISA)을 이용한 유전자 재조합 히루딘의 정량

• 영문명: Quantitation of Recombinant Hirudin by Enzyme-Linked Immunosorbent Assay
• 발행기관: 대한약학회
• 저자명: 최윤주(Yun Joo Choi) 한범수(Bum Soo Hahn) 안미영(Mi Young Ahn) 박병근(Pyung Keun Park) 손정훈(Jung Hoon Shon) 최의성(Eui Sung Choi) 이상기(Sang Ki Lee) 김영식(Yeong Shik Kim)
• 간행물 정보: 『약학회지』제41권 제1호 (1997년), 74~80쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1997.01.31

영문 초록

A polygonal antibody against recombinant hirudin was raised for the development of a ELISA in biological fluids. Recombinant hirudin was conjugated to maleimide activated carrier protein, KLH and injected to a rabbit. The third booster collection of antiserum was used as primary antibody for the ELISA. The titer for the detection antibody was determined. The direct ELISA could determine the concentration of hirudin in the range of ~10ng/ml. Affinity pulified IgG was obtained and conjugated to horseradish peroxidase. Purified IgG and IgG-HRP could be used as capture and detection antibody, respectively. Although sandwich ELISA would not give the satisfactory results. it could apply for the detection of hirudin level in the range of ~20mcg/ml.

키워드

ELISA hirudin thrombin inhibition.