L1210 및 HL60 Cell에 대한 연교의 세포독성 성분

• 영문명: Cytotoxic Constituents from the Forsythiae Fructus against L1210 and HL60 cells
• 발행기관: 대한약학회
• 저자명: 이준성(Jun Sung Lee) 민병선(Byung Sun Min) 배기환(Ki Hwan Bae)
• 간행물 정보: 『약학회지』제40권 제4호 (1996년), 462~467쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1996.08.31

영문 초록

Forsythiae Fructus was studied on cytotoxic activities for the purpose of finding out active consituents against L1210 and HL60 cells. To isolate the active ones, the methanolic extract was partitioned into water insoluble and water soluble fractions. Furthermore, the water soluble fraction was fractionated into four parts, n-hexane, benzene, ethylacetate and water fractions. Among these, the water insoluble fraction showed the most potent cytotoxic activities on L1210 and HL60 cells in vitro. The water insoluble fraction was applied to silica gel column chromatography and divided into 5 fractions(fr. 1-5). The active constituents I and II were isolated from fr.2 and 3, respectively, by repeated silica gel column chromatography and recrystallization. The constituents were identified as 3beta-acetylbetulinic acid and betulinic acid by means of physicochemical data. The ED50 values of 3beta-acetylbetulinic acid and betulinic acid were 9.10 and 16.43mcg/ml against L1210 cells and 2.72 and 2.41mcg/ml against HL60 cells, respectively.

키워드

Forsythiae Fructus cytotoxic activities L1210 cells HLGO cells 3β -acetylbetulinic acid betulinic acid.