녹수초의 항암활성물질의 분리 및 항암력 평가

• 영문명: Isolation and Evaluation of an Antitumor Constituent from Pyrolae Herba-
• 발행기관: 대한약학회
• 저자명: 배기환(Ki Hwan Bae) 김환묵(Hwan Mook Kim) 이상명(Sang Myung Lee)
• 간행물 정보: 『약학회지』제40권 제2호 (1996년), 225~229쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1996.04.30

영문 초록

The cytotoixic effect of Pylorae Herba (Pyrola japonica Klenze) against L1210 and K-562 cells was studied in vitro. The methanolic extract of Pylorae Radix was added to the culture of L1210 cells and K-562 cells for the cytotoxic activity and the ED50 values of hexane, ethylacetate, buthanol and water fractions from methanolic extract were determined using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide) assay. The active constituent isolated by bioassay guided fractionation followed by purification gave rise to a yellow needle crystal and was clarified to be chimaphilline by the comparison with the published data. The average life spans with it were not prolonged significantly on tumor growth in hybrid female mouse (BDF1-KIST) inoculated subcutaneously with P388 cells.

키워드

Pyrolae Herba Pyrola japonica L1210 cells K-562 cells P-388 cells cytotoxic activity hybrid mouse (BDFl-KIST) antitumor activity.