형질전환에 의한 S.cattleya의 카바페넴 항생제 생산성 향상

• 영문명: Improvement of Carbapenem Antibiotics Productivity in S. cattleya by Transformation
• 발행기관: 대한약학회
• 저자명: 박지선(Ji Sun Park) 이강만(Kang Man Lee)
• 간행물 정보: 『약학회지』제40권 제2호 (1996년), 212~217쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1996.04.30

영문 초록

Streptomyces cattleya is a producer of carbapenem antibiotics, thienamycin and N-acetylthienamycin, which have potent and broad-spectrum antibacterial activities. We studied on strain improvement for antibiotic productivity of S. cattleya by transformation technique which employed S.cattleya protoplasts and chromosomal DNAs of glutamic acid producers: Corynebacterium glutamicum and Arthrobacter simplex. 150 Transformant strains were cultured and bioassayed using Bacillus subtilis and Staphylococcus aureus as test organisms. 8.7% of transformants tested showed 1.4~2.6 fold higher productivities than wild type which produced 1.61 +/- 0.67mcg/ml. The best transformant produced 8.36 +/- 2.84mcg/ml carbapenems.

키워드

S. cattleya antibiotic transformation protoplast regeneration productivity.