DNA와 상호작용에서 T4 endonuclease V의 C-말단 부위의 역할에 관한 분광학적 연구: 핵자기공명과 형광 실험

• 영문명: The Spectroscopic Study on the Role of C-terminal Region of T4 endonuclease V in the Interaction with DNA; NMR and Fluorescence experiment
• 발행기관: 대한약학회
• 저자명: 유준석(Jun Seok Yu) 임형미(Hyung Mi Lihm) 임후강(Hu Kang Ihm) 신정휴(Jung Hyu Shin) 이봉진(Bong Jin Lee)
• 간행물 정보: 『약학회지』제40권 제2호 (1996년), 193~201쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1996.04.30

영문 초록

In order to study the role of C-terminal aromatic region of T4 endonuclease V in the interaction with substrate DNA, NMR and Fluorescence spectrum were recorded. Analysis of fluorescence emission spectra showed that C-terminal region of T4 endonuclease V is in or very near the binding site. In the HSQC spectrum of 15N-Tyr-labeled T4 endonuclease V*DNA complex, the broadening of a peak was observed. It is presumed that this peak corresponds to one among three tyrosine residues which belong to the WYKYY segment of C-terminal region of T4 endonuclease V. Interactions of peptide fragments consisting of C-terminal residues of T4 endonuclease V with DNAs(TT-, T^T-DNA) were investigated by NMR and Fluorescence experiment. The results suggest that two peptide fragments themselves bind to DNAs and their binding pattern is not an intercalation mode.

키워드

T4 endonuclease V(T4 endo V) Peptide fragment NMR Fluorescence.