새로운 HPLC용 형광유도체화제인 1-(N,N-dimethylamino)pyrene-6-sulfonyl chloride를 이용한 아민화합물의 분석

• 영문명: Determination of Amine Compounds Using 1-(N,N-Dimethylamino)pyrene-6-sulfonyl chloride as a New Fluorescent Derivatizing Reagent for HPLC
• 발행기관: 대한약학회
• 저자명: 이윤중(Yoon Joong Lee) 김용희(Yong Hee Kim) 조정길(Jeung Kil Cho)
• 간행물 정보: 『약학회지』제35권 제4호 (1991년), 288~294쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1991.08.30

영문 초록

A new fluorescent derivatizing reagent was developed to be used in HPLC for the trace determination of primary and secondary amines. This new reagent, 1-(N,N-dimethylamino)pyrene-6-sulfonyl chloride, was synthesized by the chlorination of sodium 1-(N,N-dimethylamino)pyrene-6-sulfonate which was obtained from 1-(N,N-dimethylamino)pyrene after sulfonation. Ephedrine and norephedrine were derivatized quantitatively by this reagent. The optimum conditions for the derivatization such as pH, reagent concentration, reaction time and reaction temperature ware examined. The structures of derivatives were identified by IR, 1H-NMR and MS methods. The fluorescence properties and the stability of the derivatives were examined. The derivatives were separated on silica column with an isocratic elution using the mixture of n-hexane and ethylacetate and monitored by fluorescene detector. Linear calibration curves were obtained and detection limits in a 10 mcl injection volume were 5 picomole for ephedrine and norephedrine.

키워드

1-(N,N-dimethyl amino)pyrene-6-sulfonyl chloride new fluorescent derivatizing reagent HPLC analysis of amines.