은행잎 플라보놀배당체에 대한 셀루라제류의 영향

• 영문명: The Effect of Cellulases on Flavonolglycosides of Ginkgo Leaf
• 발행기관: 대한약학회
• 저자명: 배기환(Ki Hwan Bae) 민병선(Byung Sun Min) 백흠영(Hum Young Baek) 안병준(Byung Zun Ahn)
• 간행물 정보: 『약학회지』제35권 제4호 (1991년), 271~276쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1991.08.30

영문 초록

The extractability and stability of ginkgoflavonolglycosides under presence of several cellulose preparations were investigated. The enzymes used were macerosin, cellulose C and cellulase NC. The content variation of the glycosides was measured with HPLC method, using caffeic acid as an internal standard. The methanol extract of ginkgo leaf, containing the total flavonolglycosides of 4.46%, was used for the content comparison. By extraction with the enzymes, each or mixed, the peak levels of all the glycosides began to decrease after 1 or 2 hours. After 24 hour extraction, most of the glycosides were degraded to minor components. The flavonolglycosides in ginkgo leaf were also hydrolysed simply by the water extraction. After 24 hour extraction with water at 40oC, the peak levels of major glycosides were distinctly decreased. Rutin was hydrolysed by enzyme treatment or by ginkgo leaf itself. As a result, it was concluded that the commercially available cellulases and the ginkgo leaf itself contain the activities of beta-glycosidase and alpha-rhamnosidase. Kaempferol-3-0-(6''-O-p-coumaroylglucosyl)-rhamnoside and four other ginkgo flavonolglycosides were not hydrolysed under the same condition.

키워드

Ginkgo biloba macerosin cellulase C cellulase NC hydrolysis of flavonolglycosides.