MLS계 항생물질 유도 내성 유전자의 크로닝과 유전자의 조절기전 Streptococcus sp. TR-1에서 분리한 pMB4 Plasmid의 MLS계 항생물질 유도내성

• 영문명: Cloning of the MLS Antibiotics Inducible Resistance Gene and Its Control Mechanism Inducible Resistance to MLS Antibiotics of pMB4 Plasmid Isolated from Streptococcus sp. TR-1
• 발행기관: 대한약학회
• 저자명: 정순학(Soon Hak Jeong) 곽진환(Jin Hwan Kwak) 김희선(Hee Sun Kim) 심미자(Mi Ja Shim) 최응칠(Eung Chil Choi) 김병각(Byong Kak Kim)
• 간행물 정보: 『약학회지』제34권 제2호 (1990년), 139~146쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1990.04.28

영문 초록

Streptococcus sp. TR-1 which has inducible resistance to MLS antibiotics was isolated from soil samples in Korea. Streptococcus sp. TR-1 was cultured in Lysis broth, then a plasmid was isolated by modified Elliker method. Bacillus subtilis UOTO277 was transformed with that plasmid. This result showed that the plasmid has the gene relating with inducible resistance to MLS antibiotics. It was named pMB4 and its size was determined about 2.4 Kb by results of digestion with various restriction enzymes. Restriction endonuclease cleavage site map of pMB4 plasmid was made by double digestion of the plasmid. pMB4 plasmid has different restriction endonuclease site map from the other plasmids that have been discovered in Streptococcus sp. so far. And it could be identified that pMB4 plasmid does not have homology with erm K of Bacillus licheniformis EMR but has homology with erm C of Staphylococcus aureus from the results of Southern hybridization.

키워드

MLS antibiotics resistance gene cloning Streptococcus pMB4.