백서 심실 근세포 L형 Ca2+ 전류에 대한 유체압력의 효과

• 영문명: Effect of Fluid Pressure on L-type Ca2+ Current in Rat Ventricular Myocytes
• 발행기관: 대한약학회
• 저자명: 이선우(Sunwoo Lee) 우선희(Sun-Hee Woo)
• 간행물 정보: 『약학회지』제50권 제2호 (2006년), 111~117쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2006.04.30

영문 초록

Cardiac chambers serve as mechanosensory systems during the heamodynamic or mechanical disturbances. To examine a possible role of fluid pressure (FP) in the regulation of atrial Ca2+signaling we investigated te effect of FP on L-type Ca2+ current (ICa in rat ventricular myocytes with electronically comtrolled micro-jet system. FP suppressed the magnitude of peak ICa by =~ 25% at 0mV without changing voltage dependence of the current-voltage relationship. FP significantly accelerated slow component in inactivation of ICa+but not its fast component. Analysis of steady-state inactivation curve revealed a reduction of the number of Ca2+ channels available for activity in the presence of FP. Dialysis of myocytes with high concentration of immobile Ca2+ buffer partially attenuated the FP-induced suppression of ICa+. In addition, the intracellular Ca2+ buffering abolished the FP-induced acceleeration of slow component in ICa inactivation. These results indicate that FP sup- preses Ca2+ currents, in part, by increasing cytosolic Ca2+ concentration.

키워드

fluid pressure(FP) L-type Ca2+ current(ICa+) ventricular myocytes whole-cell patch clamp Ca2+ buffer