Electrospary Tandem Mass를 이용한 혈중 글리퀴돈의 정량법 개발 및 생체이용률 시험

• 영문명: Electrospary Tandem Mass Spectrometry for the Quantification and Bioavailability Test of Gliquidone in Human Plasma
• 발행기관: 대한약학회
• 저자명: 문철진(Chul-Jin Moon) 이은희(Eun-Hee Lee) 양송현(Song-Hyun Yang) 문해란(Hae-Ran Moon)
• 간행물 정보: 『약학회지』제49권 제3호 (2005년), 212~216쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2005.06.30

영문 초록

A rapid, sensitive and selective electrospray tandem mass spectrometric (ESI-LC/MS/MS) method for the quantitation of gliquidone in human plasma was developed. A bioavailability study of gliquidone tablet (30 mg gliquidone, Boehringer Ingelheim Korea Co.) was performed using the validated ESI-LC/MS/MS method. The dose of 30 mg of gliquidone (1 tablet) was orally administered to 9 healthy Korean subjects. After administration, blood was taken at 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 5, 7, 9, 12, 24, and 33 hour. The validation data were as follows; the standard curve was linear (γ2=0.999) over the concentration range of 10~1000 ng/ml. The coefficient of variation for intra- and inter-day assay were 8.30~18.86, and 2.19~12.92%, respectively. The lower limit of quantification for gliquidone was 10 ng/ml. The pharmacokinetic parameters obtained were as follows; AUCt was 3861.17±1328.61 ng.hr/ml, Cmax was 831.02±227.99 ng/ml, tmax was 2.94±0.77 hr, Ke was 0.19±0.06 1/hr, and t1/2 was 4.47±3.52 hr. Based on the validated analytical method and pharmacokinetic parameters, a standard guideline of the bioavailability test of gliquidone dosage forms was prepared successfully and could be used for the bioequivalence test of gliquidone preparation.

키워드

electrospray tandem mass gliquidone bioavailability